Tatjana KOZLOVSKA

Tatyana KOZLOVSKA

Dr.habil.biol. Tatyana KOZLOVSKA

Latvian Biomedical Research and Study Centre

Ratsupites iela 1
Riga, LV 1067, Latvia

Phone: + 371 7808211
Fax: + 371 7442407
E-mail: tatyana@biomed.lu.lv

Born: August 12, 1955

Interests:

Protein engineering of virus-like particles
Expression of foreign genes in eukaryotic cells
Modelling of viral replication

Languages: Latvian, Russian, English

Education

University of Latvia (Faculty of Biology), 1977
Dr. biol., Institute of Molecular Biology, Moscow, 1985
Dr. habil. biol., University of Latvia, Riga, 1997.

Experience

Junior Research Fellow, Institute of Organic Synthesis, Riga, 1977 - 1981
Research Fellow, Institute of Organic Synthesis, Riga, 1981 - 1989
Research Fellow, Institute of Molecular Biology, Riga, 1981 - 1989
Senior Research Fellow, Biomedical Research and Study Centre, Riga, 1991 -

Honours and Awards

Corresponding Member, the Latvian Academy of Sciences, 2003
The Latvian Academy of Sciences Award, 1992

Professional Activities and Memberships

Member, Latvian Biochemical Society, 1991 -
Member, Association of Latvian Scientists, 1990 -

Recent/Representative Publications

Pumpen P.P., Dishler A.V., Kozlovskaya T.M., Bychko V.V., Gren E.J., Rivkina M.B., Grinberg A.P., Kukaine R.A. Cloning of hepatitis B virus DNA in Escherichia coli. - Dokl Akad Nauk SSSR, 1981, vol. 260, N 4, pp. 1022-1024.
Pumpen P., Kozlovskaya T.M., Borisova G.P., Bichko V.V., Kalis J., Kukaine R.A., Gren E.J. Expression of hepatitis B virus surface antigen gene in E.coli - Gene, 1984, vol. 30, pp. 207-216.
Kozlovskaya T.M., Pumpen P.P., Borisova G.P., Dishler A.V., Bychko V.V., Kalis J.V., Gren E.J., Kukain R.A. Synthesis of the full amino acid sequence of the surface antigen of the hepatitis B virus in Escherichia coli. - Dokl Akad Nauk SSSR, 1984, vol. 274, N 5, pp. 1250-1253.
Kozlovskaya T.M., Pumpen P.P. Structure and expression of human hepatitis B virus surface antigen. - Mol. Biol. (Mosk), 1986, vol. 20, N 4, pp. 884-901.
Kozlovskaya T.M., Pumpen P.P., Dreilina D.E., Tsimanis A.J., Ose V.P., Tsibinogin V.P., Gren E.J. Formation of capsid-like structures as a result of expression of the cloned gene of the envelope protein of the RNA-containing bacteriophage fr. - Dokl Akad Nauk SSSR, 1986, vol. 287, N 2, pp. 452-455.
Borisova G., Bundule M., Grinstein E., Dreilina D., Dreimane A., Kalis J., Kozlovskaya T., Loseva V., Ose V., Pumpen P., Pushko P., Snikere D., Stankevica E., Tsibinogin V., Gren E.J. Recombinant capsid structures for exposure of protein antigenic epitopes. - Mol.Gen. (Life Sci. Adv.), 1987, vol. 6, pp. 169-174.
T.Kozlovska, I.Cielens, D.Dreilina, A.Dislers, V.Baumanis, V.Ose, P.Pumpens. Recombinant RNA phage Qb capsid particles synthesized and self-assembled in E.coli. - Gene, 1993, vol. 137, pp. 133-137.
P.Pushko, T.Kozlovskaya, I.Sominskaya, A.Brede, E.Stankevica, V.Ose, P.Pumpens, E.Grens. Analysis of RNA phage fr coat protein assembly by insertion, deletion and substitution mutagenesis. - Protein Engineering, 1993, vol. 6, pp. 883-891.
Kozlovska T.M., Cielens I., Vasiljeva I., Strelnikova A, Kazaks A, Dislers A, Dreilina D, Ose V, Gusars I., Pumpens P. RNA phage Qb coat protein as a carrier for foreign epitopes. - Intervirology, 1996, vol. 39, pp. 9-15.
Kozlovska T.M., Cielens I., Vasiljeva I., Bundule M., Strelnikova A., Kazaks A., Dislers A., Dreilina D., Ose V., Gusars I., Pumpens P. Display vectors. II. Recombinant capsid of RNA bacteriophage Qb as a display moiety. - Proc. Latv.Acad.Sci., 1997, vol. 51, N 1-2, pp. 8-12.
Vasiljeva I., Kozlovska T., Cielens I., Strelnikova A., Kazaks A., Ose V., Pumpens P. Mosaic Qb coats as a new presentation model. - FEBS Letters, 1998, vol. 431, pp. 7-11.
Cielens I, Ose V, Petrovskis I, Strelnikova A, Renhofa R, Kozlovska T, Pumpens P. Mutilation of RNA phage Qß virus-like particles: from icosahedrons to rods. - FEBS Letters, 2000, vol. 482, N 3, pp. 261-264
Forsell K., Xing L., Kozlovska T., Cheng R.H., Garoff H. Membrane proteins organize a symmetrical virus. - EMBO J 2000, vol. 19, pp. 5081-5091.
A.Zajakina, T.Kozlovska, R.Bruvere, J.Aleksejeva, P.Pumpens, H.Garoff. Translation of hepatitis B virus )HBV) surface proteins from the HBV pregenome and precore RNAs in Semliki Forest virus driven expression. - J.Gen.Virol., 2004, vol. 85, pp. 3343-3351.
T.Kozlovska, A.Zajakina, V.Ose, R.Bruvere, J.Aleksejeva, P.Pumpens, H.Garoff. Synthesis of all Hepatitis B structural proteins in Semliki Forest virus expression system. - Acta Universitatis Latviensis, 2004, vol. 676, pp. 39-51.
I.Sominskaya, E.Alekseeva, D.Skrastina, V.Mokhonov, E.Starodubova, J.Jansons, M.Levi, A.Prilipov, T.Kozlovska,V.Smirnov, P.Pumpens, M.G.Isaguliants. Signal sequences modulate the immunogenetic performance of human hepatitis C virus E2 gene. - Mol. Immunology, 2006, vol. 43, N 12, pp. 1941-1952.
S.Braun, A.Zajakina, E.Alekseva, A.Sharipo, R.Bruvere, H.Meisel, P.Pumpens, T.Kozlovska. Proteasomal deegradation of HBV core protein from naturally occuring internally deleted core gene variants. - J. Clin. Microbiol., 2006 (submitted).

Research Projects:

National

T. Kozlovska (Head of the Project). Self-assembly of virion-like structures in eukaryotic cell cultures. Latvian Council of Science (1994 - 1996).

T. Kozlovska (Participant). Construction of recombinant Treponema pallidum surface antigen for diagnostic purposes. Ministry of Education and Science (1996).
T. Kozlovska (Head of the Project). Reconstruction of viral particles in vivo. Latvian Council of Science (1997 - 2000).
T. Kozlovska (Participant). Universal Cloning and Expression System by Means of Protease Mutants. Latvian Council of Science (1997 – 2000).
T. Kozlovska (Participant). Pathologies associated with the undiagnosable forms of the hepatitis B virus. Latvian Council of Science (1998 - 2001).
T. Kozlovska (Participant). New age diagnostics of the sexually transmitted diseases. Latvian Council of Science (1998 - 2001).
T. Kozlovska (Head of the Project). Reconstruction of viral particles in vivo. Latvian Council of Science (2000 - 2003).
T. Kozlovska (Participant). RNA bacteriophage Qß coat mutants with exchanged AB loop. Ministry of Education and Science (2001).
T. Kozlovska (Participant). Molecular mechanisms of variability and persistence of hepatitis viruses. Latvian Council of Science (2002 - ).
T. Kozlovska (Head of the Project). Construction of HBV pseudovirions in cultivated eukaryotic cell lines. Ministry of Education and Science (2003 - ).
T. Kozlovska (Head of the Project). Modelling of Replication of HCV and Other RNA Viruses. Latvian Council of Science (2004 - ).

International

T. Kozlovska (Participant). Recombinant viral particulate proteins as tools for new vaccines and diagnostics. INCO RTD project ERB-CIPA-CT-0187.
T. Kozlovska (Participant). Development of a novel episomal vector based on papillomaviral replicons and its use in gene therapy. INCO RTD project ERB-CIPA-CT-

T. Kozlovska (Participant). Particulate carriers in the development of AIDS vaccines and therapy. EC Concerted Research Programme EVA

T. Kozlovska (Contractor). Hepatitis B virus assembly, structure and entry into new host cells. KIRT programme

T. Kozlovska (Contractor). Expression, purification and structure determination of ENA-78. KIRT Programme

T. Kozlovska (Participant). Research and development of immunological methods. production of ELISA tests for the diagnosis of hepatitis B. NATO Science for Stability

T. Kozlovska (Participant). Expression, purification and structure determination of ENA-78. The Royal Swedish Academy of Sciences, Cooperation between Sweden and the former Soviet Union.

T. Kozlovska (Contractor). DNA vaccination against hepatitis C virus infection. INCO-Copernicus RTD project, IC 15980313

T. Kozlovska (Participant). Molecular monitoring and pathological role of HCV, HGV and altered HBV genomes in Baltic region. INCO-Copernicus RTD project, IC 15980319

T. Kozlovska (Participant). Combined immune and gene therapy for chronic viral hepatitis. QoL RTD Project, QLK2-Ct-2000-01476

T. Kozlovska (Participant). Artificial chimeric proteins as diagnostic, vaccine, and gene therapy carriers. QoL Project, Marie Curie Training Site QLK2-1999-51115

T. Kozlovska (Participant). Construction of HBV/HCV replication models for the design and screening of putative antiviral drugs. Taiho Project.

T. Kozlovska (Participant). Rational design and comparative evaluation of novel genetic vaccines. Framework 6 Integrated Project "COMPUVAC".

Last update 12.04.2007